Production of Bovine Collagen Hydrolysate with Antioxidant Activity; Optimized by Response Surface Methodology

Antioxidants are widely used in pharmaceutical industries. Gelatin is a byproduct of the meat industry and its hydrolysates showed several functionalities, such as antioxidant activity. The purpose this study was to describe optimize enzymatic hydrolysis conditions including time, temperature, pH, enzyme/substrate ratio (E/S) produce protein hydrolysate with functionality from bovine gelatin by RSM; scavenging activity evaluated using DPPH method. model observed fitted desirable adequacy sufficiency. We found that increased significantly (p < 0.05) increase pH value, E/S ratio, time process; however, temperature had no significant effect on hydrolysate. optimum were at 35.3 °C, 8.0, 2.5 after 2 h trypsin enzyme. results under these conditions, optimized RSM, could be more effective Regarding potential, can an supplement